Rebecca Mauriella, 061324153002 (2017) Kadar HSP 70 dan Kortisol pada Serum Sapi Pejantan Sebelum dan Sesudah Pengambilan Semen. Thesis thesis, Universitas Airlangga.

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Abstract

Artificial Insemination is a breeding technique by injecting semen into the female genital tract by using Artificial Vagina. The process of collecting semen, bulls can experience of stress which the effect can be affect in reproduction process. This study aims to prove that the increased levels of HSP 70 and cortisol in bull’s serum after collecting semen as an indicator of stress in cattle. Blood serum used for ELISA (Enzyme-Linked Immunosorbent Assay) test using Bovine-HSP 70 Test Kit and Bovine Cortisol Test Kit then the result read by ELISA reader. Data were analyzed using T-Test by SPSS (Statistical Programs for Social Scientific). The result showed that no significant differences. The conclusion from this study was that elevated levels of HSp 70 and cortisol levels were not significant, so it is suggested further research on the increased levels of HSP 70 and cortisol levels to the manufacture of frozen semen for artificial insemination.

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