FARAH AIDAH NURREZA, 061211133038 (2016) PROFIL PROTEIN BAKTERI Pseudomonas aeruginosa DENGAN METODE SODIUM DODECYL SULPHATE POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE). Skripsi thesis, Universitas Airlangga.

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Abstract

The aim of this research to know the protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).The side purpose of this research to give a basic knowledge about immunogenic serodiagnostic test for Pseudomonas aeruginosa. This research started by isolate Pseudomonas aeruginosa bacteria to Nutrient Agar, Mac Conkey Agar, Blood Agar for 24-48 hours and 370C. Pseudomonas aeruginosa identification used microscopic examination and biochemical tests. Pseudomonas aeruginosa colonies that grew in Nutrient Broth will be extracted using sonication process to gain whole protein from Pseudomonas aeruginosa. Sonication process used ultrasonic homogenizer 20.000 hz 4x4 minutes with 2 minute interval. Supernatant from sonification process will denaturated by Sodium Dodecyl Sulphate (SDS) for 5 minutes 1000C and electrophoresis process with 200 volt for 2 hours. The characterization by SDS-PAGE showed that Pseudomonas aeruginosa have six bands were 34 kDa; 39 kDa; 47 kDa; 49 kDa; 59 kDa and 66 kDa.

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