MUHAMMAD NAHIL FAHSYA, 061211133103 (2016) PENGARUH INSULINE LIKE GROWTH FACTOR-I (IGF-I) SERUM KUDA BUNTING CROSSBREED DIBANDINGKAN DENGAN INSULINE-LIKE GROWTH FACTOR-I (IGF–I) RECOMBINANT TERHADAP JUMLAH ANAK MENCIT (Mus musculus). Skripsi thesis, Universitas Airlangga.

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Abstract

This study was conducted to prove the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) compared with IGF-I recombinant mouse on litter size in mice (Mus musculus). The subject of this research were 35 female mice and 21 male mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were K0 = 0.1 ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, P3 = 40 ng/ml of IGF-I PMS, P4 = 10 ng/ml of IGF-I recombinant mouse, P5 = 20 ng/ml of IGF-I recombinant mouse, and P6 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include litter size in mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference), by SPSS 21 for Windows program with a significance level of p<0,05. The result effect of Insulin- Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) and IGF-I recombinant mouse on litter size in mice (Mus musculus) did not significantly difference (p>0,05). Different test results indicate that there are significant differences between the addition 0.1 ml of physiological NaCl with 40 ng/ml of IGF-I PMS.

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