TIARA RIZKA FITRIANTY, 061211132096 (2016) DETECTION OF Escherichia coli RESISTANCE TO AMPICILLIN, AZTREONAM, CEFPODOXIME, AND CEFEPIME WHICH IS ISOLATED FROM CHICKEN MEAT. Skripsi thesis, Universitas Airlangga.

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Abstract

This research aims to detect the presence of Escherichia coli in chicken meat and its resistance towards beta-lactam antibiotics; Ampicillin, Cefpodoxime, Aztreonam, and Cefepime. Isolation and identification of Escherichia coli was done by semi-MPN test using BGBB medium, EMBA medium, and Indole test to identified the presence of Escherichia coli. The result from isolation and identification test was from twenty out of twenty samples were positive contaminated with Escherichia coli. The antibiotic susceptibility test was done by using Kirby Bauer’s disk diffusion method in Muller Hinton Agar (MHA). Based on this research result, all samples were found positive contaminated with Escherichia coli, and the susceptibility test result shown complete resistance to Ampicillin and Cefpodoxime. Four samples (20%) were resistant, 13 (65%) samples were intermediately susceptible, and 3 (15%) samples were susceptible to Aztreonam 30μg. One (5%) sample was intermediately susceptible, and 19 (95%) samples were susceptible to Cefepime 30μg.

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