PENGARUH LATIHAN DAYA TAHAN DENGAN INTENSITAS MODERAT TERHADAP EKSPRESI INTEGRIN SEBAGAI PENANDA EPITHELIAL-TOMESENCHYMAL TRANSITION TROFOBLAS SAAT AKHIR MINGGU PERTAMA KEBUNTINGAN PADA MENCIT (Mus musculus) Repository

PENGARUH LATIHAN DAYA TAHAN DENGAN INTENSITAS MODERAT TERHADAP EKSPRESI INTEGRIN SEBAGAI PENANDA EPITHELIAL-TOMESENCHYMAL TRANSITION TROFOBLAS SAAT AKHIR MINGGU PERTAMA KEBUNTINGAN PADA MENCIT (Mus musculus)

Herdiantri Sufriyana, NIM011514153011 (2017) PENGARUH LATIHAN DAYA TAHAN DENGAN INTENSITAS MODERAT TERHADAP EKSPRESI INTEGRIN SEBAGAI PENANDA EPITHELIAL-TOMESENCHYMAL TRANSITION TROFOBLAS SAAT AKHIR MINGGU PERTAMA KEBUNTINGAN PADA MENCIT (Mus musculus). Thesis thesis, Universitas Airlangga.

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Abstract

BACKGROUND Pre-gestational endurance training (PGET) with chronic, moderate-intensity is promising method to prevent preeclampsia by improving epithelial-to-mesenchymal transition (EMT) of trophoblasts. PURPOSE The main objective of this study was to demonstrate the effect of PGET on α5- and α6-integrin expressions as EMT markers of trophoblasts at the end of first week gestation in mice. METHODS Five-to-six weeks old female BALB/c mice (n=32) were randomly allocated to PGET (n=16) and control (n=16) group. In PGET group, mice swam with moderate intensity for 30-minute-limited duration each day in 28 days. Gravid uterus were randomly obtained from six mice each group at the seventh day gestation or embryonic day 7 (E7). The tissue sections were analyzed via immunohistochemistry either for α5- or α6-integrin expressions at mesometrial region. RESULT In the PGET group, either the count of α5-integrin immunostained nuclei cells (α6-integrin cytotrophoblasts [CTBs] in human), or the count of α6- integrin ones (α5-integrin extravillous trophoblasts [EVTs] in human), were significantly lower than control group (p<0,01). Higher spatial difference of α6:α5 expression ratio in the PGET group compared to control group was demonstrated (p=0.014). CONCLUSION The PGET induced more distinguished EMT by changing integrins repertoire of trophoblasts. Meanwhile, smaller gravid uterus indicated by lower overall cell counts in placenta region compared to control group could give relatively less hypoxic condition in the rest of gestation as well as potentially inducing intrauterine fetal growth restriction.

Item Type:

Thesis (Thesis)

Additional Information:

KKA KK TKD 17/17 Suf p

Uncontrolled Keywords:

Mice, physical endurance, exercise therapy, integrins, epithelialmesenchymal transition

Subjects:

R Medicine

Divisions:

01. Fakultas Kedokteran

Creators: