IGA WAHYU ADITYA, 061211131044 (2016) EFEKTIFITAS PERENDAMAN EKSTRAK Spirulina platensis SEBAGAI IMUNOSTIMULAN TERHADAP JUMLAH MELANOMACROPHAGE CENTERS LIMPA IKAN GURAME (Osphronemus gouramy) YANG DIINFEKSI Aeromonas hydrophila. Skripsi thesis, Universitas Airlangga.

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Abstract

The aim of this research was to investigate the immunostimulantpotentitial of Spirulina platensis extract against disease-causing bacteria Aeromonas hydrophilato feature the histopathology by decreasing the splenic melanomacrophage centers. The research method was an experimental laboratory with five treatments (n=5) and four repetitions. Twenty gouramy fishes with the average weight 20-30 grams were randomly divided into five groups, including P0-, P0+, P1, P2 and P3 respectively for control (P0-), the group that infected with 106ml-1Aeromonas hydrophila(P0+) and the groups that immersedin water containtSpirulina platensis extract at 200, 400 and 600 mg L-1 and infected with 106 ml-1Aeromonas hydrophila. After one week of adaptation, P1, P2 and P3 groups were immersed with Spirulina platensis extract on day 1 and 7, and then infected with 106ml-1Aeromonas hydrophila. After 4 days of infection, all fish were euthanazed for data collection. The data were analyzed with Kruskal Wallis test. This research showed there were significant differences (p<0.05) between groups either for number of melanomacrophage centers (MMC). It was proved that P2 and P3 were effective dose, that showed the most mild number of melanomacrophage centers (MMC).

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