Lestari, Wong Aihuwa Diah and Suherni Susilowati and Warsito, Sunaryo Hadi (2014) The Effect Various Thawing Time on Frozen Semen of At Tailed Sheep For Viability, Motility and Intact Plasma Membrane Of Spermatozoa. Ovozoa e – Journal, 3 (2). pp. 247-251. ISSN 2302 – 6464

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Abstract

The research was aimed to determine the effect and the best thawing time on frezen semen of fat-tailed sheep for viability, motility and intact plasma membrane spermatozoa. This research was conducted at the Teaching Farm Airlangga University, with type of research is true experiment in a Completely Randomized Design (CRD). Each group consisted of 3 treatments and 6 replication. The sample in this study were frozen semen of fat-tailed sheep. The sample criteria used was fat-tailed sheep among 4 years old male, with weight 50 kg. The independent variable in this study was the thawing time of the frozen semen of fat-tailed sheep containing thawing time P1 = 15 seconds, P2 = 30 seconds, P3 = 45 seconds. The dependent variable was the quality of fat-tailed sheep’s spermatozoa. Then the data result processed using ANOVA oneway and continued with Honestly Significant Difference (HSD) test. Based on the result, it’s indicated that the difference thawing time showed a tangible influence againts (p<0,05) againts the percentage of viability, motility and intact plasma membrane. Highest average percentage obtained by 52,5% for viability, 33,3% for motility and 44,5% for intact plasma membrane on P2 treatment and lowest percentage obtained from P1 treatment with 36,8% for viability, 17,5% for motility and 33,3% for intact plasma membrane

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