ROSALIA FRISKA ANANDA, 051311133025 (2017) UJI AKTIVITAS EKSTRAK ETIL ASETAT DAUN Cassia spectabilis TERHADAP PROSES PENGHAMBATAN DETOKSIFIKASI HEME. Skripsi thesis, Universitas Airlangga.

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Abstract

Cassia spectabilis is traditionally used as an antimalarial drug. In the previous study, it is known that in vitro methanol extract on C. spectabilis leaves had antimalarial activity with IC50 value of 2,66 μg / ml, while IC50 value of ethyl acetate fraction IC50 was 1,88 μg/ml and IC50 of chloroform fraction was 0,642 μg ml. Based on TLC results, it is known that alkaloids content in the extract was greater than the content of other classes of compounds and thus it was expected to have antimalarial activity (Ekasari, et al., 2012). This study aimed to determine the activity of ethyl acetate extract of C. spectabilis leaves in the process of inhibiting heme detoxification and to detect the class of compounds in the extract based on TLC. The method used for antimalarial activity test was of Basillico et al (1998) method that had been modified. The concentration of extract used is 4; 2; 1; 0,5; 0,25; and 0,1 mg/ml. The absorbance value was measured using an ELISA reader at a wavelength of 405nm. The inhibitory activity of heme detoxification was expressed in IC50 using a probit analysis. The results of this study indicated that ethyl acetate extract of C. spectabilis had inhibitory activity against heme detoxification with IC50 value of 0,333 mg/ml (KV = 3,604%) and based on the TLC analysis of ethyl acetate extract of C.spectabilis leaves, it was showed that alkaloids, flavonoids, and terpenoids were detected.

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