ULFA, 051311133249 (2017) PENETAPAN KADAR (–)-EPIGALOKATEKIN GALAT DALAM PRODUK TEH HITAM DENGAN METODE KROMATOGRAFI CAIR KINERJA TINGGI. Skripsi thesis, Universitas Airlangga.

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Abstract

Tea (Camellia sinensis) has many benefits for human due to its high content of catechin especially EGCG. It has antioxidant activity 100 times more effective than vitamin C and 25 time more effective that vitamin E. Based on their preparation processes, tea were differentiated into several kinds such as green tea, black tea, white tea and oolong tea. Black tea is the most consumed tea in the world by 78%. This study aimed to determine EGCG concentrations in black tea product with HPLC method. The samples were selected from several products on the local market. The HPLC condition were as follow, column was used RP C-18 μBondapak 10μm, 3,9 x 300 mm column. The mobile phase was methanol: water:acetic acid 2% (35:60:5 v/v/v) isocratically. The flow rate was 0,45 mL/minute and the wavelength for detecting the samples was 276,0 nm with Diode Array Detector. The method was validated for selectivity, linearity, precision, accuracy, limit of detection, and limit of quantitation. Based on dry weight of the sample, it was found that the EGCG concentrations in black tea (A), black tea (B) and black tea (C) were 0,047 ± 0,002 % ; 0,132 ± 0,017 % and 0,162 ± 0,026 % of dry weight respectively. Based on the results, the HPLC method can be used to determine the EGCG concentrations in black tea products.

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