KIKI LESTARI, 051311133036 (2017) PENGARUH MEDIA DENGAN SUMBER KARBON AGRO RESIDUES TERHADAP AKTIVITAS ENZIM FIBRINOLITIK Bacillus megaterium BM 9.1. Skripsi thesis, Universitas Airlangga.

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Abstract

The study effect of agro residues medium as a carbon sources on fibrinolytic enzyme activity produced by Bacillus megaterium BM 9.1 was done by using fibrin plate method. Bacillus megaterium BM 9.1 grown on agro residues medium with Solid State Fermentation. The fibrinolytic enzymes activity is declarated by fibrinolytic index. Fibrinolytics index is the comparisson between clear zones and wall diameters. From various carbon sources tested, Bacillus megaterium BM 9.1 which is grown in banana peel, pineapple skin, corn husk, rice bran and corn cob could produce fibrinolytic enzymes. Optimum fibrinolytic activities found in medium combination nutrient agar and banana peel at fibrinolytic index 4,95 ± 0,08 (p<0,05). If compare with nutrien agar is 117,58 %. Optimation concentration of banana peel was done at concentration of 0,5 %, 1 %, 2 % and 4 % and the fibrinolytic index was found respectively according to 4,26 ± 0,11 ; 4,32 ± 0,03 ; 4,61 ± 0,03; and 4,35 ± 0,05. The optimum fibrinolytic activity was found in medium banana peel consentration 2 %.

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