AFRILIA DIANA FITRI, 051311133075 (2017) PENGARUH SUMBER KARBON ORGANIK TERHADAP PRODUKSI PROTEASE FIBRINOLITIK Bacillus sphaericus BM 9.1 DENGAN SOLID STATE FERMENTATION (SSF). Skripsi thesis, Universitas Airlangga.

Preview	Text (ABSTRAK) FF.KF.39.17 . Fit.p - ABSTRAK.pdf Download (98kB) | Preview
	Text (FULLTEXT) FF.KF.39.17 . Fit.p - SEC.pdf Restricted to Registered users only until 1 November 2020. Download (2MB) | Request a copy

Abstract

Fibrinolytic enzymes are a group of protease serine enzymes capable of destroying blood clots (fibrin) in various thrombotic diseases. Fibrinolytic enzyme sources can be obtained from microorganisms. Bacillus sp is one of the microorganisms that has the potential to produce fibrinolytic enzymes. Medium has an important role in growing and producing fibrinolytic enzymes. In a medium, sufficient carbon source is required for the growth of a bacterium. In microbial fermentation, carbon sources are indispensable for the formation of biomass and energy production. The medium used in this research is Nutrient agar medium with organic carbon source added. Sources of carbon used are glucose, fructose, sucrose, and starch with each 1% w/v concentration. Addition of carbon sources with 1% w/v concentration to the medium showed different effects on fibrinolytic enzymes production by Bacillus sphaericus BM 9.1. So among all the carbon sources tested, fructose yielded the highest fibrinolytic index (2.86 ± 0.03). The next step is to optimize the concentration of fructose carbon source. Optimization of fructose concentration to be tested at concentrations of 1%, 2%, 3%, and 4%. All concentrations has been tested, fructose 1% w/v concentration yielded the highest fibrinolytic index (2.82 ± 0.04).

Actions (login required)

View Item