Diane Lukito Setiawan, dr, NIM011318156302 (2017) PENGARUH γH2AX DAN PERSENTASE SEL NK TERAKTIVASI TERHADAP DERAJAT APOPTOSIS PADA PASIEN LEUKEMIA AKUT DEWASA. Thesis thesis, Universitas Airlangga.

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Abstract

Apoptosis is programme cell death which can inhibit cell proliferation of acute leukemia. Phosphorylated H2AX (γH2AX),is a response to DNA damage, that can lead to the activation of p53 protein and simultaneously activates NK cells to induce apoptosis in patients with acute leukemia. This study aimed to assess the effect of γH2AX and activated NK cells to apoptosis in patients with acute leukemia. Methods and materials. This is a cross-sectional observational study design. Subjects consisted of 21 newly diagnosed acute leukemia patients who were treated at the Department of Internal Medicine, Dr. Soetomo Hospital-Surabaya from December 2016 - March 2017. γH2AX and apoptosis (89-kDa fragment PARP) is being tested using specimens from peripheral blood mononuclear cells (PBMC), while NK cell activity by using whole blood specimens. Examinations were done by flowcytometry (BD FACSCaliburTM). The results were statistically analyzed using path analysis with linear regression techniques. Results.The mean of γH2AX, the percentage of activated NK cells and the degree of apoptosis in patients with acute leukemia was 2.27% ± 1.63%, 2.99% ± 1.88%, and 2.52% ± 1.73%. Respectively, these results showed significant differences with normal adults. There were no influences of γH2AX for NK cells activation (p = 0.851), γH2AX to apoptosis (p = 0.591), and activated NK cells to apoptosis (p = 0.432).

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