dr. Shafira Nadia (2017) PENGARUH PRAKONDISI HIPOKSIA TERHADAP EKSPRESI HYPOXIA INDUCIBLE FACTOR (HIF) 1-α, B-CELL LYMPHOMA-2 (BCL-2), DAN MAMMALIAN TARGET OF RAPAMYCIN (mTOR) PADA KULTUR ADIPOSED-DERIVED MESENCHYMAL STEM CELL (AMSCs). Thesis thesis, Universitas Airlangga.

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Abstract

myocardial damage by exploring the pluripotency of stem cells for regeneration and cardiovascular repair. But low proliferation and low survival, remains a major obstacle to clinical use. Various stress conditions such as exposure to oxidative stress during isolation can lead to apoptosis. Therefore we need a strategy to optimize this stem cell therapy. Precondition strategies with hypoxia to enhance AMSCs activity appear as a safer and clinically appropriate alternative strategy to increase viability of stem cells, and reduce cell damage and apoptosis under the stimulation of ischemic conditions, as well as increase proliferation and self-renewal in stem cells Objective: To demonstrate the benefits of hypoxic precondition in cultures of adipose-derived mesenchymal stem cells (AMSCs) against AMSCs survival and angiogenic capabilities represented by increased HIF-1α, BCL-2 and mTOR expression. Methode: This study was conducted in vitro true experimental posttest only with control group design on AMSCs culture. AMSCs isolated from lipoaspirate (minimally invasive) were then identified AMSCs first by looking at CD90 +, CD105 + and non-expression CD45 expression - then cultured up to the 4th cell. The result of this culture was then harvested and made into 16 samples per group. The samples were then divided into 2 groups of cultures ie normoxia culture group (control) with 21% oxygen concentration and treatment group (P1) with 1% oxygen concentration where both groups were cultured within 24 hours. After that, samples were then observed HIF-1α expression, and mTOR expression using immunofluorescence method and BCL-2 expression using immunocytochemical method. The experimental results are determined by the distribution of the data and then tested different tests to see the significance.

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