ANDIKA INDRA KUSUMANINGRUM, 061424253006 (2017) PERBANDINGAN SENSITIVITAS PRIMER BERBEDA PADA METODE Polymerase Chain Reaction ( PCR ) UNTUK DETEKSI INFEKSI Trypanosoma evansi ISOLAT BANTEN PADA TIKUS PUTIH ( Rattus novergicus ). Thesis thesis, Universitas Airlangga.
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Abstract
Trypanosoma evansi is the causative agent of the disease surra in Indonesia. This disease could affect productivity and health. Parasitological, serological and molecular methods are mostly used for detecting the infection. The aim of this study was to evaluate primers sensitivity (ITS1 and TBR1/2) for detecting the parasite on rats infected with T. evansi isolate collected from Banten province in 2014. In addition, the data of PCR results were compared to other two methods, native blood tests and Card agglutination test for Trypanosoma (CATT). A total of 18 rats were divided into three groups i.e. uninfected rats (negative control), infected rats with 101 of T. evansi and infected rats with 104 of T. evansi. The rats were observed everyday for two weeks. The blood of rats were collected every day for PCR analysis and every two days for native blood test after infection. A serological test was conducted every four days after infection. The result demonstrated that for the infected group with 101, T. evansi was detected on 66.66% (4/6) rats at day three and 33.33% (2/6) at rats at day one for the ITS 1 primer and the TBR1/2 primer, respectively. Meanwhile, for the infected group with 104, T. evansi was detected on 83.33% (5/6) rats at day three and 50.00% (3/6) at rats at day one for the ITS 1 primer and the TBR1/2 primer, respectively. However, the navite blood test showed positive response at day four and the serological result (CATT test) provided negative response until at the end of observation for both infected groups (101 and 104). In conclusion, the TBR1/2 primer was more sensitive than the ITS1 primer because the TBR1/2 primer was able to detect the parasite earlier than the native blood test and CATT technique. The primer can be used for detecting the parasite in the pre patent period of T. evansi infection.
| Item Type: | Thesis (Thesis) | |||||||||
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| Additional Information: | KKC KK TPKMV.11/17 Kus p | |||||||||
| Uncontrolled Keywords: | TBR1/2 and ITS1 Primers, PCR, Sensitivity, Trypanosoma evansi | |||||||||
| Subjects: | S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine | |||||||||
| Divisions: | 06. Fakultas Kedokteran Hewan > Ilmu Penyakit dan Kesehatan Masyarakat Veteriner | |||||||||
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| Depositing User: | Mr Binkol1 1 | |||||||||
| Date Deposited: | 31 Dec 2017 01:04 | |||||||||
| Last Modified: | 31 Dec 2017 01:04 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/66723 | |||||||||
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