ZAFITRI NURYATI WASTOMI, 061424253008 (2018) KARAKTERISASI PROFIL PROTEIN ANTIGENIK LARVA CACING Anisakis spp. YANG MENGINFEKSI IKAN TONGKOL (Euthynnus sp.) PENELITIAN EKSPLORATIF LABORATORIS. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The purpose of this research to perform protein analysis and characterization on third stage larvae of Anisakis spp. which can stimulate the formation of antibodies to obtain an antigenic protein profile so it can be utilized for the development of diagnostics in cases of anisakiasis. Samples of third stage larvae Anisakis spp. taken from the gastrointestinal tract of mackerel tuna fish (Euthynnus sp.) from the Brondong Auction Market (TPI) of Brondong, Lamongan. Third stage larvae Anisakis spp. has been collected then crushed and added PBS to make homogenate and then synthesized and centrifuged at 5000 rpm for 5 min. The homogenate of Anisakis spp. was immunized to rabbit at a dose of 500 μg / rabbit subcutaneously with booster 4 times at 2 weeks intervals. The homogenate of Anisakis spp. was performed using the SDS-PAGE technique to analyze the protein with Coomassie blue staining. Antigenic protein of Anisakis spp. was characterized using by western blot technique and obtained 6 protein bands with molecular weight (BM) are 64 kDa, 45 kDa, 38 kDa, 30 kDa, 27 kDa and 23 kDa.

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