TIKA AULIA PUTRI, 061414253016 (2018) IDENTIFIKASI GEN PENYANDI MecA BAKTERI Methicillin-resistant Staphylococcus aureus DARI SWAB TANGAN MAHASISWA PPDH DI RUMAH SAKIT HEWAN UNIVERSITAS AIRLANGGA PENELITIAN EKSPLORATIF LABORATORIS. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The research was done with the aim to identify the mecA gene Methicillin-resistant Staphylococcus aureus (MRSA) from PPDH students swab hand. In total, 10 hand swab samples from coass students were collected and analysis. Bacterial identification based on the growth in MSA media, Gram staining, catalase test, coagulase test, hemolysis test, screening test with ChromID MRSA, the antibiotic sensitivity test with Oxacillin, and mecA genes by using Polymerase Chain Reaction (PCR). Isolation and identification Staphylococcus aureus were found in three samples that showed positive result in MSA, positive catalase test, positive coagulase test, and positive hemolysis test. MRSA screening test were found in three samples that showed positive MRSA strain. Antibiotics sensitivity test for three of samples found that Staphylococcus aureus in 33,33% resistant and 66,7% sensitive to Oxacillin. In PCR test, one sample showed positive presence of mecA gene. In conclusion, the presence of mecA gene was found and it could be indicated by direct contact with pets or owners with infected MRSA.

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