DINDA DEWI ARTINI, drg, 021518036301 (2018) PERBEDAAN LAMA PENYINARAN PHOTODYNAMIC THERAPY TERHADAP JUMLAH BAKTERI Enterococcus faecalis(Penelitian Eksperimental Laboratoris). Thesis thesis, Universitas Airlangga.

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Abstract

The goal of endodontic treatment is to eliminate pathogenic bacteria of infected root canals. The most frequent bacteria can cause infected root canals is Enterococcus faecalis which is resistant to some medicaments and root canal irrigation. Photodynamic therapy (PDT) is a new antimicrobial method that involves the use of a photosensitizer and a light source for root canal disinfection. Irradiation time of PDT can affect produce of singlet oxygen and ROS to destruct bacteria Purpose : To analyze difference irradiation time of photodynamic therapy against the number of Enterococcus faecalis bacteria Method : This study used a culture of Enterococcus faecalis bacteria which was divided into 7 groups in eppendorf tubes. Group I as the control group, group II, III, IV, V and VI was given photosensitizer Toluidine Blue O (TBO) and photoactivated with irradiation time for 10, 20, 30, 40, 50 and 60 seconds. After incubation the number of bacteria was calculated. Result : There was significance difference (p < 0,05) among the various groups. Irradiation time of PDT for a longer time (Group VI and VII) showed the significantly higher antibacterial effects of Enterococcus faecalis bacteria. Conclusion : The more irradiation time of PDT results the less number of Enterococcus faecalis bacteria. A 50-second irradiation time is the most effective time to against the entire number of this bacteria.

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