Tania, Saskianti and Rianita, Ramadhani and Els S, Budipramana and Seno, Pradopo and Ketut, Suardita (2017) Potential Proliferation of Stem Cell from Human Exfoliated Deciduous Teeth (SHED)in Carbonate Apatite and Hydroxyapatite Scaffold. Journal of International Dental and Medical Research, 10 (2).

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Abstract

Stem cell from Human Exfoliated Deciduous Teeth (SHED) is a source of stem cells that is easy to obtain. The application of SHED can be used for repairing damaged teeth, bone regeneration, and treatment of neural tissue injury. Scaffold can be used as a carrier for application of SHED and also increase the cell growth and regeneration of damaged tissue. Carbonate apatite and hydroxyapatite are material which commonly used as scaffold. The aim of this study was to evaluate the potential proliferation of SHED in carbonate apatite scaffold and hydroxyapatite scaffold as candidate biomaterials in bone tissue engineering. SHED was obtained from dental pulp of primary extracted teeth. SHED was seeding in carbonate apatite and hydroxyapatite scaffold for 7 days. The proliferation of SHED were determined using Scanning Electron Microscope (SEM). The average number of SHED was higher in carbonate apatite scaffold compared to hydroxyapatite scaffold. Combination of SHED and carbonate apatite scaffold has higher proliferation potency compare with SHED and hydroxyapatite. Experimental article (J Int Dent Med Res 2017; 10(2): pp. 350-353) Keywords: SHED, carbonate apatite, hydroxyapatites, scaffold.

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