Widjiati and Epy M. Luqman and Firman Kristianto Sumedi and Ismudiono and Nanik Sianita (2009) Karakterisasi Growth Differentiantion Factor 9 (GDF 9) pada Oosit Sapi yang Dikoleksi dari Folikel Preantral dengan Metode Dotblotting. Veterinaria Medika, 2 (1). pp. 13-16. ISSN 1979-1305

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Abstract

The main purpose of this research was to know the character of Growth Differentiation Factor-9(GDF-9) protein isolated from bovine oocyte, collected from preantral follicle. Bovine ovary that was collected from slaughter house washed with NaCl. To collect oocyte from preantral follicle we use aspiration technique. The GDF-9 protein was isolated from oocyte and used for producing the antibody for dotblotting method. The rabbits which had been injected by GDF-9 antigen (immunization) was bleed every week to getthe blood serum and five week after immunization, the rabbits had boosted again and continued to bleed until10 week. The antibody anti-GDF-9 from the rabbits blood serum was reacted with GDF-9 antigen that had been identified with western blotting method. The dot-blot result showed that the intensity of dot color had increased significantly from bleeding III (five week after injected with antigen GDF-9) and reach the highest titer of antibody at bleeding IX. It’s mean that GDF-9 protein from bovine oocyte which collected frompreantral follicle can be characterized with dotblotting method and the highest titer antibody showed at bleeding IX

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