DEA PARAMITHA UTAMI, 061324253008 (2018) PEMBUKTIAN GEN CylE PADA Streptococcus agalactiae YANG DI ISOLASI DARI SUSU SAPI YANG MENDERITA MASTITIS SUBKLINIS DI SURABAYA PENELITIAN EKSPLORATIF LABORATORIS. Thesis thesis, Universitas Airlangga.

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Abstract

The aim of this research is to isolate, identification, and determine fragment gene CylE in Streptococcus agalactiae from bovine subclinical mastitis milk. This research use 94 samples of cow's milk. The results showed of 94 samples of cow's milk obtained from farms in Surabaya (Kaliwaron, Wonocolo, Bendul Merisi, Platuk and Pogot) had CMT to determine the incidence of mastitis subclinical showed 73 milk samples positive (78%) to California Mastitis Test (CMT). S. agalactiae was isolated by conventional methods Blood Agar , incubate at 37°C in 24 hours. Identification process include macroscopic, microscopic, catalase test, and CAMP test. Isolation and identification showed 4 samples are the bacterial species Streptococcus agalactiae. Fragment gene CylE was detected by Polymerase Chain Reaction and electrophoresis. The result of this study shows the whole (100%) samples bacteria Streptococcus agalactiae contains Toxin β-haemolysin / cytolysin factors encoded by ClyE. The results of electrophoresis shows the band DNA the primary amplifies CylE with the length 248 bp

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