Budiatin, Aniek Setiya and Parmanasari,, Twoti Dika and Khasanah, Uswatun and Khotib, Junaidi (2009) Effect of U-50,488 H on the Development of Drug Craving Behavioral and Protection of Morphological Change in Prefrontal Cortex, Thalamus and Amygdale of the Mice (Musmusculus) In theMorphine-Induce Dependence. Majalah Farmasi Airlangga, 7 (2). p. 4. ISSN 0852-1050

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Abstract

Repeated treatment of morphine produces several change in brain and behavior that far outlast their initial neuropharmacological effects. The nature of persistent drug-induced neural plasticity is interested because it is thought to contribute to the development of drug dependency and addiction. The purpose of this study was designed to investigate the effect administration of a selective κopioid reseptor agonist (U-50,488H) on rewarding effect associated with morphine in mice and the neuron morphological change on the several brain region (PFC,thalamus and basolateral amygdala). An unbiased conditioned place preference was developed to evaluate the rewarding effect of morphine in this animals. Drug craving model was induced by subcutant injection of morphine at 3 mg/kg BW( based on previous study) once a day for six altermately days. Various doses U-50,488H ( 0.1 mg/kg; 1.0 mg/kg; 3mg/kg BW) was administered by subcutant injection pretreatment with morphine. The data showed that rewarding effect of morphine was strongly suppressed by pretreatment U-50,488H and significantly attenuated at dose 0.1 mg/kg (F (4.48) = 14.024;p<0.05). The brain tissue was harvested on day 11 and sliced stereotaxically on PTC, thalamus and basolateral amygdale. The slicing was stained with routine histology staining, hematoxyllin eosin for morphological qualitative analysis. The result showed that U-50,488H restore neuron morphology in the several brain region (PFC, thalamus and basolateral amygdale) induced by morphine. Keywords : Conditioned Place Preference; drug craving; morphine; PFC, thalamus and basolateral amygdale; U-50,488H

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