Wahyuni, Dwi Kusuma and Hafida, Siti Nur and Ermayanti, Tri Muji and Eko Wardoyo, Bambang Prajoga and Purnobasuki, Hery and Wida Utami, Edy Setiti (2017) Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510. Biosaintifika Journal of Biology & Biology Education, 9 (1). pp. 26-32. ISSN 2085-191X

There is a more recent version of this item available.

Preview	Image C 12. J. Biosaintifika.9 (1); 26-32 (2017). p-ISSN 2085-191X; e-ISSN 2338-7610. (Anggota)..pdf Download (138kB) | Preview
	Text C 12. J. Biosaintifika.9 (1); 26-32 (2017). p-ISSN 2085-191X; e-ISSN 2338-7610. (Anggota)..pdf Restricted to Registered users only Download (285kB)
Preview	Text (Similarity) 12. Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510.pdf Download (3MB) | Preview
Preview	Text C 12. Validasi.pdf Download (4MB) | Preview

Abstract

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD 600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD 600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction.

Available Versions of this Item

• Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510. (deposited 02 Mar 2022 02:26) [Currently Displayed]
  • Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510. (deposited 25 Jun 2019 07:41)

Actions (login required)

View Item