Wiwik Misaco Yuniarti, - and Didik Handijatno, - and Emy Koestanti Sabdoningrum, - and Wiwiek Tyasningsih, - (2017) Detection of OMP-31 Gene Encoding Brucella Suis’s Local Isolates OMP-31 Protein with Polymerase Chain Reaction. Journal of Applied Environmental and Biological Sciences, 7 (1). pp. 49-52. ISSN 2090-4215

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Abstract

Swine brucellosisi is a zoonosis affecting pigs, caused by the bacterium Brucella suis, and an economically important cause of reproductive losses in pigs. In humans, brucellosis can be a serious, debilitating and sometimes chronic disease that may affect a variety of organs. In pigs, B. suis occurs in the fetus, placenta, fetal fluids and vaginal discharges after an abortion or stillbirth. This study used aborted fetus as sample. For the isolation of Brucella suis, material was inoculated on sterile brucella broth and then on plates of brucella selective agar media. The isolates suspected for Brucella suis were subjected to Gram staining and Stamp’s modified Ziehl-Neelsen (MZN) staining for confirming the purity of cultures and morphological characters. Pure suspected Brucella isolates, were analysed for their biochemical profiles for the differentiation of Brucella species on the basis of biochemical tests. The results showed and assuring that the isolates obtained were B. suis. PCR assays resulted in the amplification of 723-bp bands from the targeted omp31 genes of the Brucella suis as a local isolates. Well-established immuno dominant outer membrane protein (31 kDa omp) can be a milestone for the development of effective diagnostic to eradicate the disease. KEYWORDS: B. suis, omp31, PCR, 723 bp

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