Aulia Charis Aqsha, 051411131050 (2018) PEMURNIAN DAN KARAKTERISASI METABOLIT FRAKSI-4 EKSTRAK ETIL ASETAT JAMUR ENDOFIT Aspergillus salwaensis DTO297C1 YANG DIISOLASI DARI Chromolaena odorata. Skripsi thesis, Universitas Airlangga.

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Abstract

The endophytic fungi Aspergillus salwaensis DTO297C1 were isolated from Chromolaena odorata. The activity showed positive antimicrobial against 3 tested bacteria namely B.subtillis, S. aureus, E. coli, and fungus C. albicans. After fractionation, fraction-4 also demonstrated similar activities. Further research was carried out for the metabolite purification and characterization of ethyl acetate extract fraction-4 of endophytic fungus Aspergillus salwaensis DTO297C1. Purification was carried out using preparative thin layer chromatography method, stationary phase silica gel 60 GF254, mobile phase n-hexane: ethyl acetate = 2: 6. The results of purification were 10 subfractions. Subfraction was further characterized using GC-FID, HP-5 column. The result of GC-FID showed subfraction to be further characterized were subfraction 4.4, 4.7, 4.8, and 4.10. The GC-MS method was chosen for further characterization of the three subfractions. There were compounds identified in the three subfractions, i.e. penicillic acid, ethanone,1-(2-hydroxy-4-methoxyphenyl), stigmasterol, γ- sitosterol, β-sitosterol, and pinostrobin.

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