ASTIN MIRANTI KUSUMA INDRAJATI, 051411131022 (2018) EKSPRESI mRNA VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT α2δ-1 DI SUMSUM TULANG BELAKANG PADA MENCIT DENGAN NYERI NEUROPATI YANG DIINDUKSI OBAT PACLITAXEL. Skripsi thesis, Universitas Airlangga.

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Abstract

Paclitaxel has mechanism to inhibiting disassembly of tubulin from microtubule polymere, also improve Substance P and calcitonin gene-related peptide (CGRP) in plasm and bronchoalveolar lavage fluid. Generally, voltage-dependent calcium channel (VDCC) is one of the most important Ca2+ regulators in neurons, including physiological nociception and chronic pain/neuropathy. The presence of a chemotherapy drug may alter expression of the VDCC α2δ-1 subunits in the dorsal root ganglion (DRG) and possible alteration of α2δ-1 subunit expression in spinal cord. The groups consisting of the control group and the neuropathic pain group model induced 12 mg/Kg of paclitaxel. Mice were sacrifice every week, i.e. 1, 2, and 3 weeks after paclitaxel injection, then spinal cord (L4-6) were taken. After obtaining spinal cord, total RNA was extracted, then converted mRNA to cDNA and amplified with polymerase chain reaction (PCR) to obtain PCR product. PCR product was applied in a 1,7% agarosa gel. Samples separated on 1,7% agarose gel and visualized with Ethidium Bromide. Then documented with Gel Documentation under UV light to see the sample band. The results of the study there were α2δ-1 mRNA expression in spinal cord induced 12 mg/Kg BB paclitaxel and upregulated in spinal cord after injection of 12 mg/Kg BB paclitaxel in the third week.

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