AJENG NISA KARIMAH OKTAVIA, 061411131022 (2018) PENGARUH PENAMBAHAN TEPUNG JANGKRIK KALUNG (Gryllus bimaculatus) DALAM PAKAN MENCIT (Mus musculus) JANTAN TERHADAP JUMLAH SEL SPERMATOGENIK DAN SEL SERTOLI. Skripsi thesis, Universitas Airlangga.

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Abstract

The aim of this research was to examine the effect of the ‘Kalung’ cricket’s (Gryllus bimaculatus) flour addition in male mice (Mus musculus) feed toward the amount of spermatogenik cells’ and sertoli cells’. The effect of cricket flour was tested on 20 mice that are randomly divided prior to this research. K (control) was given the standard feed, P1 was given the standard feed with the addition of 5% cricket flour, P2 with the addition of 10% cricket flour, and P3 with the addition of 20% cricket flour. The treatment was done for 45 days, then the mice were sacrificed. Testes sample was taken by conducting surgery, and the calculation of spermatogenik cell’s and sertoli cells’ amount were done by using Optilab and Image Raster software with 400x magnification. The result was analyzed using Analysis of Variant (ANOVA) and continued with Duncan's double-spaced test. Spermatogenik cells’ amount are 40,1 ± 1,7 (K), 58,0 ± 11,5 (P1), 32,7 ± 2,5 (P2), 30,4 ± 1,6 (P3). Sertoli cells’ amount are 4,52 ± 1,00 (K), 4,72 ± 1,22 (P1), 4,54 ± 0,82 (P2), 2,32 ± 0,46 (P3) which shows the significant differences (P<0.05). Therefore, the conclusion is, the addition of 5% cricket’s flour is the optimal dose to increase spermatogenik cells’ amount and maintain sertoli cells’ amount.

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