WERENFRIDUS KONO LAKE, 061411535003 (2018) UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK n-HEKSANA DAN KLOROFORM DAUN SIRSAK (Annona muricate L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA In Vitro. Skripsi thesis, Universitas Airlangga.

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Abstract

This study aimed to determine the antibacterial activity of soursop leaf against Staphylococcus aureus. The extraction was processed by maceration method first with n-hexane then chloroform. The antibacterial activities were measured in vitro by means of agar diffusion method using paperdisk. The antibacterial activity was tested by analysis of variance (ANOVA) with a significance value of 0.5% to know which treatment had an effect or significantly different with a dose respectively 300 mg / ml, 250 mg / ml, 200 mg / ml, 150 mg / ml, 100 mg / ml and produce antibacterial activity with consecutive inhibition zone of 16,70 mm; 14,05 mm; 11,45 mm; 9,85 mm; 3,00. The results of antibacterial activities examination showed chloroform extract can inhibited the growth of Staphylococcus aureus at concentration 250 mg/ml with inhibitation zone diameter were 14.05 mm. Extract with the highest activity determined the minimum inhibitory concentrations grow (MIC). MIC soursop leaf chloroform extract of the Staphylococcus aureus bacteria that was at a concentration of 100 mg/ml with inhibition zone of 3.00 mm.

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