Elsya Aurellia Yonanda, NIM011511133222 (2018) EFFICACY OF BENZALKONUM CHLORIDE AGAINST: Multidrug-resistant Acinetobacter baumannii. Skripsi thesis, Fakultas Kedokteran.

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Abstract

The prevalence of Healthcare-associated Infections (HCAI) in hospitals is alarming (5-12% in developed countries & 5-19% in developing countries). Most of them are caused by bacteria which more likely to be resistant to several types of antibiotic, for example Acinetobacter baumannii. It has been speculated resistance to disinfectant may contribute to the epidemiology of the organism in clinical settings. This study’s aim is to find the bactericidal effect of benzalkonium chloride against multidrug-resistant Acinetobacter baumannii (MDR-Ab). This study was categorized as true experimental method MDR-Ab specimens were obtained from Microbiology Laboratory Faculty of Medicine Universitas Airlangga, Surabaya. Benzalkonium chloride was prepared with concentration as follows: 80 μg/ml, 40 μg/ml, 20 μg/ml, 10 μg/ml, 5 μg/ml, 2.5 μg/ml, 1.25 μg/ml, 0.625 μg/ml, 0.3125 μg/ml. Dilution test with Nutrient broth medium were done to determine minimum inhibitory concentration (MIC). After 24 hours of incubation, isolated bacteria inside the tube was subcultured in Nutrient agar plate medium to determine the minimum bactericidal concentration (MBC). Replication of this experiment were conducted 3 times according to Federer’s formula. MIC of benzalkonium chloride to MDR-Ab was determined at 80 μg/ml. Meanwhile, MBC was also determined at 80 μg/ml. The effective concentration of benzalkonium chloride to MDR-Ab is 80 μg/ml. Further research is needed to determine the therapeutic and side effects of benzalkonium chloride against MDR-Ab in vivo. Similar studies with a wider range of bacteria and concentration is also needed to know the bactericidal spectrum of benzalkonium chloride.

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