Rafi’u Hafidz Arrasyid Sukarno, 051411131007 (2018) Pengembangan Metode Penentuan Ascorbic Acid Equivalent (AAE) Menggunakan KLT-Bioautografi Pada Ekstrak Etanol 96% Kunyit (Curcuma longa Linn.). Skripsi thesis, Universitas Airlangga.

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Abstract

Antioxidant activity by DPPH assay could be applied on uv vis spechrophotometry and TLC-Bioautography. UV-Vis spectrofotometry commonly used for determination all of antioxidant activity on sample test using DPPH asssay. Therefore, TLC-Bioautography applied to detect specific constituents in sample that have antioxidant properties . Recently, determination of antioxidant activityby measuring AA value as parameter had been developed. mustofa reported that demethoxycurcumin and curcumin had antioxidant activity by measuring AAE value on TLCBioautography but he didn't compared the value of TLC-bioautography and UV - Vis Spechtrophotometry. Their compararation can be used for prove that there isn't significant result based on statistical analysis. For determination AAE on TLC-Bioautography, two types of eluent system were used. There are Hexane:ethyl acetate and Chloroform:methanol. while determination AAE on Spectrophotometry used IC50 calculation method. The result of average value AAE on TLC-Bioautography using Hexane:ethyl acetate yielded 27,34% curcuminoid. While for Chloroform:methanol yielded 15,29% bisdemetoxycurcumin; 24,62% desmetoxycurcumin; 46,19% curcumin. The result of average value AAE on spectrophotometry yielded 51,20% from IC50 calculation. One way ANOVA sattistical test use to compare AAE value on KLTBioautography and spectrofotometry . The resulted in p value > α = 0,05, (p value 0,082). Therefore, it can be concluded that TLC - Bioautography is a valid method that can be used for determination of antioxidant activity of multicomponent compound by measuring AAE value.

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