FRESYNANDIA KARYNEISA PUTRI, 021618036320 (2018) EKSPRESI TUMOR NECROSIS FACTOR-α DAN ALKALINE PHOSPHATASE AKIBAT INDUKSI KOMBINASI KALSIUM HIDROKSIDA DAN PROPOLIS. Thesis thesis, Universitas Airlangga.

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Abstract

Background: Calcium hydroxide, the golden standart for pulp capping treatment was known to have some disadavantages such as high solubility and formation of tunnel defect. Propolis with its anti-inflammatory properties is expected to improve the treatment outcome when it is combined with calcium hydroxide as direct pulp capping agent. Objective: Evaluating of the effect of calcium hydroxide and Propolis extract combination as a direct pulp capping agent based on its capability for antiinflammation which is represented by expression of TNF-α and ALP in perforated rat teeth model. Material and Methods: This experimental study using a total of 60 maxillary first molar of wistar rat which then randomly divided into experimental combination of calcium hydroxide and Propolis extract group, control calcium hydroxide group, and negative control group. The cavities then sealed with Cention.At third days and seventh days, rats then sacrificed from each group, and sections of the teeth were obtained. After being decalcified, specimens underwent histological evaluation under light microscope to identify the presence of odontoblast-like cell and inflammation cells. The immunohistochemistry (IHC) method using anti-TNF-α and anti-ALP was then performed to evaluate the expression of TNF-α and ALP. The results then statistically evaluated by Shapiro Wilk, Homogenity test, ANNOVA tests and Tukey HSD. Results: Combination of calcium hydroxide and Propolis extract group shows most cells expressing both TNF-α and ALP, followed by control calcium hydroxide group, and negative control group as the least group expressing TNF-α and ALP . Conclusion: Propolis extract combined with calcium hydroxide, complement its function as direct pulp capping agent as their combination antiinflammation rather than the use of calcium hydroxide alone.

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