FAILA SHOFA AMARILDA, 021511133059 (2019) DAYA ANTIBAKTERI EKSTRAK KITOSAN DARI BUBUK SQUID PEN TERHADAP Streptococcus mutans. Skripsi thesis, Universitas Airlangga.

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Abstract

Chitosan is a natural material that can be obtained from fishery waste such as crustacean shells and squid pens obtained from squid. Squid pen is a waste produced from the processing of squid with transparent structures with feather-like shapes attached to the dorsal inside a squid mantle. Chitosan from squid pen has been developed in the medical field because it has biocompatible properties, biodegradable, non toxic, and also has antifungal activity. Being able to eliminate the Streptococcus mutans bacteria which is a predisposing factor for the occurrence of denture stomatitis due to the use of long-term dentures. Purpose: This study aims to determine the bacterial activity of chitosan extracted from squid pen against Streptococcus mutans. Methods: Streptococcus mutans was planted in BHIB media tubes and given chitosan suspension 1.5% (w/v) with each sample group consisting of 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78% and there are two control groups namely positive and negative controls. All samples were incubated for 24 hours at 37℃. Then a visual observation of turbidity on liquid BHIB media was then carried out by planting culture on Nutrient agar media with 7 replications, incubated for 2x24 hours at 37 ℃ and counted the number of living colonies. The results were analyzed using Oneway Anova and Post hoc tukey test. Result: At the concentration of 6,25% there was no bacterial growth in nutrient agar media. Conclusion: Chitosan that extracted from squid pens has antibacterial activity against Streptococcus mutans bacteria with minimal concentration 6,25%.

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