IRMASMITA TASNIADARA, 021618036315 (2018) DEGRADASI EEXTRACELLULER POLYMERIC SUBSTANCE BIOFILM BAKTERI Streptococcus mutans AKIBAT PAPARAN LASER DIODA 405 NM. Thesis thesis, Universitas Airlangga.

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Abstract

Backround: Streptococcus mutans (S.mutans) is a major cause of dental caries with biofilm formation. These bacteria are able to form an eksopolisakarida matrix in dental biofilms. Biofilm is a collection of microorganisms attached to a surface and covered with extracellular matrix produced by these cells fromtheir environmet. Photodinamic therapy is an alternative to eliminate bacterial biofilm. Purpose : This study used S.mutans, dioda laser 405 nm as a light source and clorophyll as exogenous photosentitizer. Six different groups were analyzed: control group, chlorophyll group, chlorophyll + laser 75”, chlorophyll + laser 90”, chlorophyll + laser 105”, and chlorophyll + 120”, The EPS was determined by using Confocal Laser Scanning Microscope (CLSM). Result : Groups with laser and chlorophyll showed significant EPS degradation compared to control group and chlorophyll group (p<0.05). Different irradiation time resulted in significant EPS degradation compared to each other (p>0.05). Conclusion : Photodynamic therapy with diode laser 405nm and chlorophyll could degrade S.mutans’s EPS.

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