IRAWAN PANDU BUDITOMO, 091515053002 (2019) TERAPI FOTODINAMIK LASER DIODA DENGAN PENAMBAHAN DOXYCYCLINE UNTUK MENINGKATKAN IMUNITAS PADA TIKUS PERIODONTITIS. Thesis thesis, Universitas Airlangga.

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Abstract

The aim of this study is to prove that the photodynamic therapy using combination of diode laser and antibiotic can enhance the immune system on periodontitis rats. In addition, we compared the periodontitis rats immune systems on four days of given therapy. In order to periodontitis modelling in rat, we injected Porphyromonas gingivalis (P.g.) in first molar region with 14 days period time. The histopathological measuring showed that the periodontitis rats is characterized by higher macrophage (7,67±0,58-10,00±2,00 cells), lymphocytes (5,00±1,00-5,67±1,53 cells), and lower fibroblast (18,33±2,31-21,67±3,21 cells) which were followed by the increased alveolar bone destruction since the first day resulting increased distance between cemento-enamel junction and alveolar bone (CEJ-AV) on last day observation (893,61±316,78-1123,23±170,60 μm). Diode laser is used as monochromatic light source which harmless with low output power using 8 J/cm2 energy density. Doxycycline can be used as photosensitzer because of the appropriately absorbance spectrum with the 405 diode laser. Statistical test results showed there was immunomodulatory effect on each control group, followed by decreasing CEJ-AV distance. The combined diode laser and doxycycline group had shown the best immunomodulatory effects and followed by tissue remodelling since the first day of applied therapy. Statistical analysis showed there are decreased macrophages (3,33±1,15 become 2,00±0,00 cells) and increased fibroblasts (30,00±4,00 become 32,33±3,05 cells) cells number, so well decreased of CEJ-AV distance (695,96±135,35 become 538,27±99,84 μm) after the last day of applied therapy. We concluded that the photodynamic therapy by combining laser diode and doxycycline can enhance the immunity on periodontitis rats.

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