FARAH FANISSA, 061411133017 (2019) DETECTION AND ISOLATION OF Salmonella sp. ON BEEF AT TRADITIONAL MARKETS IN KABUPATEN SITUBONDO. Skripsi thesis, Universitas Airlangga.

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Abstract

This study aims to detect the presence of Salmonella sp, on beef sold at traditional markets in Kabupaten Situbondo. By using the superficial part of M. gastrocnemius, 31 sample pieces was taken randomly from 14 traditional markets in 3 different regions in Kabupaten Situbondo for then to be tested referred to SNI 2897:2008 and ISO 6897:2002. Through the pre-enrichment process, each sample was rinsed by using Pepton Water medium and continued with enrichment in TetraThionate Broth Base medium (TTB). The colonies in TTB were then isolated using SS (Salmonella Shigella) agar. Growth of colorless bacterial colonies by showing the presence or absence of black spots for then carry out the re-culture process to obtain pure isolates of Salmonella sp.. After the isolation processes is done, a typical bacterial colonies were then identified and confirmed by using TSIA, Urea agar, SIM and MR-VP. Through a series of testing processes, showed that there is one sample (3.22%) positively contaminated with Salmonella sp. from 31 pieces of the total samples that used for.

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