GHEA AYU PUTRI ANGGRAENI, 051411131068 (2019) UJI AKTIVITAS ANTIMIKROBA EKSTRAK n-HEKSANA, ETIL ASETAT DAN ETANOL 96% Caulerpa racemosa ASAL PULAU MAMBURIT KANGEAN TERHADAP Eschericia coli, Staphylococcus aureus DAN Candida albicans. Skripsi thesis, Universitas Airlangga.

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Abstract

Marine organism such as seaweed or algae, are known as the source of bioactive metabolites especially Caulerpa racemosa. Previous studies showed that extract of C. racemosa from various seas in the world, including Indonesia has antimicrobial activity. The aim of this study is to investigate the antimicrobial activity of the ethanol 96%, ethyl acetate, and n-hexane extract of Caulerpa racemosa from Mamburit Island Kangean Sumenep against Eschericia coli ATCC 8739, Stahylococcus aureus ATCC 6538 and Candida albicans ATCC 10231. The antimicrobial activity was determined using disc diffusion and microdilution methods. MTT (3-(4,5-dimetiltiazol-2-il)-2-5-difenil tetrazolium bromide) reagent was added in the microdilution method to visualize the presence of living organism. The results showed that only ethyl acetate extract of C. racemosa inhibited the growth of E. coli ATCC 8739, S. aureus ATCC 6538 and C. albicans ATCC 10231 at concentration 5000 ppm (100 μg/disk) and 1000 ppm (20 μg/disk). The minimum inhibitory concentration (MIC) against E. coli ATCC 8739 and C. albicans ATCC 10231 were 500 ppm (50 μg/well), while S. aureus ATCC 6538 was 1000 ppm (100 μg/well). Phytochemical screening showed Caulerpa racemosa contained terpenoid, polyphenols, flavonoid. Antioxidant activity was positive.

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