SINGGIH PRATAMA WIYOSO, 061511133052 (2019) EFEKTIFITAS VAKSIN OUTER MEMBRANE PROTEIN 52 kDa DALAM MENCEGAH KERUSAKAN INSANG IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI DENGAN Aeromonas hydrophila. Skripsi thesis, Universitas Airlangga.

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Abstract

The aim in this study was to know effect of the vaccine of Outer Membrane Protein 52 kDa in preventing damage of nile tilapia’s gills that was infected by Aeromonas hydrophila bacteria. Twenty of nile tilapia with average weight of 40 gram and approximately length of 10-12 cm were randomly devided into four group of treatment (P0(-), P0(+), P1 and P2) and five repetition. P0(-) (control, not vaccinated and not infected), P0(+) (not vaccinated and then infected by Aeromonas hydrophila)., P1 (nile tilapia was vaccinated whole cell protein “Hydrovac®” and then infected by Aeromonas hydrophila), P2 (nile tilapia was vaccinated Outer Membrane Protein 52 kDa and then infected by Aeromonas hydrophila). Histopathologic observed was lamella part which found lamellar oedema, lamellar hyperplasia, lamellar fusion and necrosis were analyzed by Kruskal Wallis test indicates that there are significant differences (p<0.05) among treatment, the further test result by using Man-Whitney. The results of the study showed level of damage in the P2 lower than those P0(+) and P1. This proved that P2 effective can reduce damage in gills of nile tilapia.

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