AGESTI VEVA KALISTA, 061511133202 (2019) UJI SITOTOKSISITAS EKSTRAK DAUN KEMBANG BULAN (Tithonia diversifolia) TERHADAP BONE MARROW MESENCHYMAL STEM CELL (BMSC) TIKUS DENGAN METODE MTT ASSAY. Skripsi thesis, Universitas Airlangga.

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Abstract

Background: Kembang bulan leaf extract (Tithonia diversifolia) belong to Asteraceae family, they are worldwide known for their biological properties such us anti-diabetic, anti-parasitic, anti-microbial, anti-inflammatory, etc. a class of terpenoid from this plant known as sesquiterpene lactone taking the role for their biological properties and their toxicity. Purpose: The aim of this study was to identify the cytotoxicity effect of kembang bulan leaf extract (Tithonia diversifolia) on bone marrow mesenchymal stem cell using MTT assay. Methode: The sample of this research using bone marrow mesenchymal stem cell of rats. The kembang bulan leaf extract (tithonia diversifolia) divide into 4 concentration (0,5%, 0,25%.0,125%, and 0,0625%) and given to bone marrow mesenchymal stem cell of rats in each well and incubated for 24 hours. The toxicity result was obtained using MTT assay method and incubate for 4 hours. The optical density absorbency which indicates cell viability calculated by ELISA reader with a wavelength of 595nm. Cytotoxicity parameters using LC50 and analyzed by probit analysis. Conclusion: the extract of kembang bulan leaf with the concentration of 0,5%, 0,25%, and 0,125% found to kill cell up to 50% and only the concentration 0,0625% of kembang bulan leaf extract with the highest cell viability. The LC50 value of kembang bulan leaf extract on bone marrow mesenchymal stem cell of rat is 0,167%.

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