Edy Setiti Wida Utami and Issirep Soemardi and Taryono and Endang Semiarti (2007) EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN. Berkala Penelitian HAYATI(Journal of Biological Researches)., 13 (1). pp. 33-38. ISSN 0852-6834

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Abstract

ABSTRACT Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i.: NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.

Item Type:	Article
Uncontrolled Keywords:	moon orchid, somatic embryogenesis, structure and developmental pattern, light microscopy
Subjects:	Q Science > Q Science (General)
Divisions:	08. Fakultas Sains dan Teknologi > Biologi
Creators:	Creators NIM Edy Setiti Wida Utami UNSPECIFIED Issirep Soemardi UNSPECIFIED Taryono UNSPECIFIED Endang Semiarti UNSPECIFIED
Depositing User:	Mr Akhmad Nurfizal Reza
Date Deposited:	25 Jun 2019 07:27
Last Modified:	25 Jun 2019 07:27
URI:	http://repository.unair.ac.id/id/eprint/83562
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• EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN. (deposited 02 Mar 2022 02:25)
  • EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN. (deposited 25 Jun 2019 07:27) [Currently Displayed]

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