Edy Setiti Wida Utami, Dra., MS and Sucipto Hariyanto, Dr. DEA (2019) In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm. Scientifica, 2019 (2019). pp. 1-6. ISSN ID. 8105138

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	Text (COVER) Cover.pdf Download (2MB)
	Text (FULLTEXT) C 2. J. In vitro seed germination & seedling germination of P. amboinensis (Scientifica, HINDAWI).pdf Download (2MB)
	Text (VALIDASI) C 2. Validasi - In Vitro Seed Germination and Seedling Development of Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm.pdf Download (2MB)
	Text (SIMILARITY) C 2. Similarity - In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm.pdf Download (4MB)
	Text SJR Q2=0.42.pdf Download (863kB)

Abstract

Phalaenopsis amboinensis, an epiphytic orchid, has a great potential for commercial exploitation in the cut-flower industry. It is difficult to propagate vegetatively as it naturally grows slowly. ,erefore, there is a need to improve the propagation methods to avoid endangering its natural populations. ,e objective of this study was to identify the best medium and organic supplements for seed germination and plantlets development of P. amboinensis. Seeds from 4-month-old hand-pollinated orchids were sown on different strengths of MS and VW culture media: Murashige and Skoog (MS), 1/2 MS, Vacin and Went (VW), and 1/2 VW. Optimum seed germination, i.e., 90.7%, was achieved on VW medium. VW medium was suitable for seedling formation and allowed 51.4% of seedling development from protocorm within 10 weeks of culture. When 15% (v/v) coconut water was added together with banana homogenate (10 g·L−1) to the VW medium, the plantlets grew to the highest length and had the highest dry weight (62.1 mmand 15.5 g, respectively). ,e roots and leaves of the plantlets grew vigorously in this medium. Plants regenerated via in vitro seed germination processes were successfully acclimatized in greenhouse conditions, and the survival rate was more than 85%.

Available Versions of this Item

• In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm. (deposited 02 Jul 2019 02:13) [Currently Displayed]
  • In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm. (deposited 26 Jun 2019 02:50)

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