Prahesty Hana Pertiwi and Bambang Sektiari Lukiswanto, NIDN. 0008116202 and Rochmah Kurnijasanti, NIDN. 0019077004 (2015) Isolasi, Identifikasi dan Penapisan Aktivitas Antimikroba Streptomyces sp. Isolat Tanah Lumpur Lapindo Sidoarjo. Veterinaria Medika, 8 (1). pp. 51-58. ISSN 1979-1305

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Abstract

This study aims to identify the Streptomyces sp. isolated from soil of the Lapindo mudflow in Sidoarjo based on it's morphology, comparing the growth profile of Streptomyces sp. soil isolates Lapindo mudflow in Sidoarjo and see the antimicrobial activity of Streptomyces sp. against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633 and Candida albicans ATCC 10231. Four samples of mud taken from Point A with radius of 200 m from the center of mudflow, Point B with radius of 500 m from the center of mudflow, Point C with radius of 1 km from the center of mudflow and Point D is sludge disposal area Porong to get Streptomyces sp. isolates. The Streptomyces sp. isolates morphology had been observed, the dry weight of the cells measure to determine the growth curve profile and conducted screening antimicrobial activity against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633 and Candida albicans ATCC 10231. This research obtained 8 Streptomyces sp. isolates, namely Streptomyces D1, D2, D4, D6, D7.1, D7.2, D9 and D12 isolated from Point D. Each of these isolates have different characteristics in tenns of colony morphology, growth profile and antimicrobial potential against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Bacillus subtifis ATCC 6633 and Candida albicans ATCC 10231. Keywords: Streptomyces sp., Lapindo Sidoarjo mudflow, antimicrobial activity

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