Suherni Susilowati, - and Trilas Sardjito, - and Oky setyo Widodo, - and Rochmah Kurnijasanti, - and Wurlina, - and Erma Safitri, - and Mustofa Helmi Effendi, - (2018) Effect of Green Tea Extract Supplementation in The semen Extender on Post-Thaw Sperm Quality of Simmental Bulls. The Philippine Journal of Veterinary Medicine, 55 (2). pp. 127-134. ISSN 0031-7705

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Abstract

The aim of this study was to determine the effects of green tea extract as antioxidant in the extender of frozen semen on the viability, motility, integrity of plasma membrane, percentage of necrosis and apoptosis of spermatozoa. Ejaculates collected from four Simmental bulls were evaluated and placed in 37°C water bath before the cryopreservation process was started. The semen samples were diluted in milk-egg yolk supplemented with green tea extract of 0 (P0) as control, 0.05 (P1), 0.1 (P2), and 0.15 (P3) mg/100 ml extender, respectively. Diluted semen packed in 0.25 ml straws were cryopreserved and stored in liquid nitrogen for four weeks. Cryopreserved semen packed in straws were thawed individually in 37°C water bath for 30 sec then evaluated microscopically. The results showed that P2 had the highest post-thaw sperm viability, motility, and integrity of plasma membrane compared to the other groups (P<0.05). In addition, the percentage of necrosis and apoptosis of sperm were lowest in P2 (P<0.05) compared to the other groups. The results of the study suggest that semen samples diluted in milk-egg yolk supplemented with green tea extract at the rate of 0.1 mg/100 ml extender had the highest semen quality. Thus, green tea extract can potentially act as antioxidant to maintain the quality of post-thaw bull spermatozoa Key words: apoptosis, bull, green tea extract, necrosis, post-thaw, sperm quality

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