Tutik Juniastuti, NIDN. 0021066604 and Rochmah Kurnijasanti, NIDN. 0019077004 (2009) The Isolation of Antibiotics Produced Streptomyces Sp. From Soil At East Java. In: Proceedings International Seminar: From Ocean for Food Security, Energy, and Sustainable Resources and Environment. Fisheries and Marine Faculty - Airlangga University and Research Center for Marine and Fisheries Socio Economics, Agency for Marine and Fisheries Research, Ministry of Marine Affairs and Fisheries, Surabaya, pp. 85-87. ISBN 978-602-96671-0-3

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Abstract

The research was done isolation of antibiotic produced Streptomyces sp.From soil of the highlands of East Java of the area of the slopes of Semeru Mountain. Land was taken from a height of 2,000m above sea level and altitude of 2,390m above sea level that had traveled lava flow of Semeru Mountain. Isolation breeds done by Streptomyces sp. Gennination medium specialized in streptomyces sp. the ISP-4.Then, perfonned by macroscopic and microscopic observations.The observations of Streptomyces sp.are macroscopically showed some characteristics:asmancolony with a diameter of 2-3mm, white, fluffy, like the skin and formhard,sporesat the end of smell miselia and provide land. By microsoopic observations, Gramstaining, isolates showed a purplish blue color, which means Streptomyces sp. was a Grarn-poSitive bacteria, into the examination with a microscope with1, 000x magnification it appears that Streptomyces sp. has slenderhifa, mycelium fonns chains with sporesand3-5 show the activity test of carbohydrates by showing a yellow color in the meclacandy. In this research can be concluded that the soil with altitude 2,000 and 2,390m above sea level there is stiDa Streptomyces sp. who survive, the expected antibiotic produced also have greater potential as an antibacterial Keywords: Streptomyces sp.,uplandsoil

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