Rochmah Kurnijasanti, - and Sri Agus Sudjarwo, - and Tutik Juniastuti, - and Martia Rani Tacharina, - (2016) Identification of Streptomyces Spp. Soil Isolate Sidoarjo Lapindo Mud Based on Gene 16S rRNA. In: International Seminar on Molecular in veterinary medicine. Faculty of Veterinary Medicine Universitas Airlangga, Surabaya, pp. 77-79. ISBN 978-602-7043-81-7

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Abstract

This study was aimed to identifY the Streptomyces sp. soil isolates of Lapindo Sidoarjo mud based on 16S rRNA gene. Several stages of research was conducted to identifY Streptomyces sp. isolates of Lap indo Sidoarjo mud based on 16S rRNA gene. The isolation of Streptomyces sp. from Lap indo Sidoarjo mud was conducted by several stages. The process was divided into isolation, purification and identification of Streptomyces sp. isolates based on its morphology. The next process was DNA isolation, purification, and identification of Streptomyces sp. isolates of lumpur Lapindo Sidoa1jo mud based on 16S rRNA gene. The isolation process resulted eight isolates of Streptomyces sp. with morphologically distinct characters. The DNA isolation and identification based on 16S rRNA gene showed 8 isolates of Streptomyces sp. of lumpur Lapin do mud has ribbon at 1500 bp long Keywords : Streptomyces sp., Lap indo Sidoarjo mud, 16S rRNA gene

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