PENGARUH pH ELUEN TERHADAP HASIL DESALTING CRUDE PERTUSSIS TOXIN (PT) DAN FILAMENTOUS HEMAGGLUTININ (FHA) DARI Bordetella pertussis

Faris Adrianto and Esti Hendradi and Isnaeni and Neni Nurainy (2014) PENGARUH pH ELUEN TERHADAP HASIL DESALTING CRUDE PERTUSSIS TOXIN (PT) DAN FILAMENTOUS HEMAGGLUTININ (FHA) DARI Bordetella pertussis. Berkala Ilmiah Kimia Farmasi, 3 (1). pp. 28-35. ISSN 2302-8270

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Official URL: http://journal.unair.ac.id/BIKF@pengaruh-ph-eluen-...

Abstract

Optimizing desalting crude process of PT and FHA using sephadex G-25 as intermediate purification phase for PT and FHA as acellular pertussis vaccine components has been developed. The factor influencing optimization including the impact of chemical factor such as eluation buffer pH towards protein’s concentrate were studied using 3 different pH (6, 7, and 8). The result were : Different Buffer pH has influence towards protein recoveries whereas phosphate buffer 50 mM pH 7.0 as eluation buffer (with 74,23 + 3,07 % recoveries percentage) best for desalting crude PT and FHA.

Item Type: Article
Uncontrolled Keywords: Desalting, Crude PT-FHA, Sephadex G-25,buffer pH, buffer concentration
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM
Faris AdriantoUNSPECIFIED
Esti HendradiNIDN0014115703
IsnaeniNIDN0013015603
Neni NurainyUNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 16 Sep 2019 01:59
Last Modified: 10 Jan 2020 05:59
URI: http://repository.unair.ac.id/id/eprint/86374
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