Dewi Melani Hariyadi and Esti Hendradi and Octavia Librayanti Vierdina Piay and Lailil Muharromah (2014) Formulasi dan evaluasi mikrosfer ca-alginat dengan model antigen ovalbumin dengan teknik aerosolisasi. PharmaScientia, 3 (2). pp. 1-10. ISSN 2302-0725

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Abstract

Ovalbumin protein was generally used as a model of vaccine antigen for oral delivery system. This research highlighted the advantages of Ca-alginate microspheres entrapping ovalbumin which prepared by ionotropic gelation using aerosolisation technique. It involved alginate as polymer and calcium chloride as crosslinker. The objective of this study was to investigate the preparation and characterization of microspheres produced from CaCl2 with different concentration and alginate in terms of size, protein loading, entrapment efficiency, and yield. The alginate concentrations used in this study were 1.5% and 2.5% w/v. While, the CaCl2 concentrations used in this study were 0.1, 0.5 and 1.5 M. The results showed that low concentration of CaCl2 could not produce microspheres, instead it produced gelling sheet. Microspheres were formed by increasing the CaCl2 concentration. Infra red spectra were also showed that crosslinked between alginate molecule and Ca2+ was occurred. Smaller microspheres was produced by increasing CaCl2 concentration from 0,5 M to 1,5 M. The effect of increasing alginate concentration from 1.5% to 2.5% w/v showed an increase of protein loading, entrapment efficiency, and yield. From particle size examination, increasing alginate concentration showed a decrease of particle size. In summary, high entrapment efficiency, high protein loading and microspheres yield, and small particle size of ovalbumin-alginate microsphere were successfully produced using the ionotropic gelation-aerosolization method which can be utilized as one of oral protein or vaccine delivery systems.

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