UMI MUFIDA, 061511133080 (2019) PENGARUH SUPLEMENTASI ESTRADIOL 17β TERHADAP PERSENTASE MATURASI IN VITRO OOSIT SAPI. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This study aimed to determine the percentage of in vitro oocyte maturation on TCM – 199 media with estradiol 17ß supplementation. Cumulus oocyte complexes (COCs) that had been selected from the dissection media were added into the maturation media. The media was using TCM-19 which were added 0,15 IU/ml PMSG, 0,15 IU/ml HCG, FBS and estradiol 17ß. Each petridish contained three drops of maturation media (300 μl/drops) which were coated with mineral oil and then incubated in a 5% CO2 incubator, with maximum humidity and temperature of 39°C. Control (P0) was oocyte maturation without supplementation with the hormone estradiol 17ß supplementation 1μg/ml. Treatment 1 (P1) was oocyte maturation with 1μg/ml of estradiol 17ß supplementation. Treatment 2 (P2) was oocyte maturation with 2μg/ml of estradiol 17ß supplementation. The calculation of mature oocyte percentage was using aceto-orcein staining. The data analyzed using ANOVA, the result showed that there were no significant differences in control (P0) 50.63%, in treatment 2 (P2) 54.07%, and in treatment 3 (P3) 48.60%. Based on the result, it can be concluded that estradiol 17ß supplementation to the maturation level of cattle oocytes showed did not effect the percentage of in vitro oocyte maturation

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