Isnaeni and Mega Ferdina Warsito and Noor Erma Nasution and Ni Made Merthaniasih and Achmad Toto Poernomo and Diana Fairuz and Astrid Hanifah (2017) Antibacterial Activity of Butanol Extract from Cell Free Fermentation Broth of Streptomyces spp.Isolated from Vegetable Plantation Soil. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 8 (1). pp. 1921-1927. ISSN 0975-8585

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	Text (SIMILARITY) Antibacterial Activity of Butanol Extract from Cell Free Fermentation Broth of Streptomyces spp.Isolated from Vegetable Plantation Soil.pdf Download (2MB)
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Abstract

Thirty five Streptomyces spp. have been isolated from vegetable plantation soil collected from Sidoarjo, East Java, Indonesia. The isolation and fermentation process to obtain antibacterial substances were performed in the International Streptomyces Projects (ISP)-4 media on rotary shaker at 150 rpm, 28oC for seven days. In vitro antibacterial properties testing of one day to five days free cell fermentation broth (CFFB) of the Streptomyces isolates have been carried out by diffusion agar method using Gram positive and Gram negative bacteria as test microorganisms. The aims of this research are to extract the CFFB of the potential isolates by n-butanol and screen in vitro anti bacterial activities of the extract. It was found that the n-butanol extract of the CFFB of Streptomyces B2 and K2 strain showed their activities against Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 12323701 with MIC of 0,1 and 0,5 ppm respectively. Streptomyces B10 and K6 strain showed their potential activities against Bacilus subtilis ATCC 6633 and Escherichia coli ATCC 25922 with MIC 0,273 and 2,93 respectively. Both isolates are also active against Mycobacterium tuberculosis H37Rv and patients isolate strain.

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