Widya Paramita Lokapirnasari and Dady Soegianto Nazar and Tri Nurhajati and Koesnoto Supranianondo and Andreas Berny Yulianto (2015) Production and Assay of Cellulolytic Enzyme Activity of Enterobacter Cloacae WPL 214 Isolated from Bovine Rumen Fluid Waste of Surabaya Abbatoir, Indonesia. Veterinary World, 8 (3). pp. 367-371. ISSN 0972-8988/2231-0916

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Abstract

Aim: This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-Dglucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia. Materials and Methods: To produce enzyme from a single colony of E. cloacae WPL 214, 98 × 1010 CFU/ml of isolates was put into 20 ml of liquid medium and incubated in a shaker incubator for 16 h at 35°C in accordance with growth time and optimum temperature of E. cloacae WPL 214. Further on, culture was centrifuged at 6000 rpm at 4°C for 15 min. Pellet was discarded while supernatant containing cellulose enzyme activity was withdrawn to assay endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase. Results: Cellulase enzyme of E. cloacae WPL 214 isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of 0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35°C and optimum pH 5. Conclusion: E. cloacae WPL 214 isolated from bovine rumen fluid waste produced cellulose enzyme with activity as cellulolytic enzyme of endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase and β-glucosidase. Keywords: endo-(1,4)-β -D-glucanase, exo-(1,4)-β-D-glucanase, β-glucosidase., Enterobacter cloacae WPL 214.

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