Suryani Dyah Astuti, NIDN. 0008046902 and Nike Dwi G. D. and Agus Supriyanto, NIDN. 0024086208 and Kuwat Triyana (2015) Photodynamic Combined with Magnetic Field Applications for Viability Activation of Anaerobic Photosynthetic Bacteria. Applied Mechanics and Materials, 771 (2015). pp. 141-144. ISSN 1660-9336 /1662-7482.

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Abstract

Abstract. This paper reports the influence of light exposure (photodynamic) combined with magnetic field application on viability activation of anaerobic photosynthetic bacteria (Rhodobacter sphaeroides). For this purpose, a customized equipment was developed. It consisted of a sample holder, arrays of light emitting diode (LED) and Helmholtz coils as light source and magnetic field source, respectively. The systems of this equipment were controlled by a microcontroller of AVR ATMega-8535. Prior to application in vitro, all arrays of LED were calibrated, both their intensity and wavelength. After the treatments, all bacteria colonies were grown in photosynthetic media (PMS) for 48 hours followed by calculating the growth of bacterial colonies using a total plate count (TPC) method and Quebec colony counter. As results, it was found that the growth of bacterial colonies was influenced by both light intensity and wavelength of LED array. At the same intensities, the wavelength of 430 nm showed highest effect on the growth of bacterial colonies. In addition, upon application of the optimum light combined with magnetic field, the highest growth of bacterial colonies was achieved more than 110% when the energy dose of light and the magnetic field were 204 J/cm2 and 1.8 mT, respectively

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