DYAH PURWITA TRIANGGADEWI, NIM011428016305 (2019) PENGARUH PEMBERIAN METFORMIN TERHADAP KADAR TRANSFORMING GROWTH FACTOR-BETA (TGF-β) DAN ALPHA-SMOOTH MUSCLE ACTIN (α-SMA) PADA SEL EPITEL LENSA MODEL FIBROSIS KAPSUL LENSA ANTERIOR SECARA IN VITRO (Studi Eksperimental Laboratorium). Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Purpose: to investigate the effect of metformin to TGF-β and α-SMA levels in lens epithelial cells (LECs) inducted fibrotic condition Methods: LECs were isolated from anterior capsule’s tissue of cataract patient. LECs were divided into 4 groups consist of FBS 2% as control group and 3 others metformin group using different doses f 0.1 mM, 0.5 mM and 1 mM. LECs was identified by characterization staining with vimentin and P63 antibodies. Confluenced cells was investigated by staining of FITC TGF-β and α-SMA antibodies and the results were observed under a fluorescence microscope. This study were analyzed oneway Anova test followed by posthoc test with significant level of p<0.05. Results: TGF-β antibody expression levels decreased after administration of metformin 0.1mM (6.96 x106 pixels ± 1.03x106pixels), metformin 0.5mM (5.35 x106pixels ± 1.23x106pixels), and metformin 1mM (1.93x106 pixels ± 0.94x106 pixels). The Tukey HSD post hoc test showed a significant decrease in expression levels in the 0.5mM metformin group (p = 0.027) and 1mM metformin (p = 0.000) compared to the 2% FBS control. The levels of α-SMA antibody expression decreased after administration of metformin 0.1mM (4.49 x106pixels ± 0.74x106 pixels), metformin 0.5mM (3.34 x106 pixels ± 0.47x106 pixels), and metformin 1mM (2.26x106 pixels ± 0.55x106 pixels). The Games-Howell post hoc test showed a significant decrease in expression levels between the control groups compared with the three treatment groups metformin 0.1mM (p = 0.035), metformin 0.5mM (p = 0.008) and metformin 1 mM (p = 0.002) compared to the FBS control 2%. Conclusion: metformin might have antifibrotic effect on LECs fibrotic model through inhibiting TGF-β and α-SMA levels expression

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